Genomic and transcriptomic analysis of the thermophilic lignocellulose-degrading fungus Thielavia terrestris LPH172

Abstract Background Biomass-degrading enzymes with improved activity and stability can increase substrate saccharification make biorefineries economically feasible. Filamentous fungi are a rich source of carbohydrate-active (CAZymes) for biomass degradation. The newly isolated LPH172 strain the thermophilic Ascomycete Thielavia terrestris has been shown to possess high xylanase cellulase activities tolerate low pH temperatures. Here, we aimed illuminate lignocellulose-degrading machinery novel in detail. Results We sequenced analyzed 36.6-Mb genome transcriptome during growth on glucose, cellulose, rice straw, beechwood xylan. 10,128 predicted genes were found total, which included 411 CAZy domains. Compared other fungi, auxiliary (AA) domains particularly enriched. A higher GC content was coding sequences compared overall genome, as well GC3 content, is hypothesized contribute thermophilicity. Primarily family 9 lytic polysaccharide monooxygenase (LPMO) glycoside hydrolase (GH) 7 glucanase encoding upregulated when cultivated cellulosic substrates. Conventional hemicellulose (GH10, GH11 various CEs), AA9 LPMOs, observed co-expression co-upregulation AA CAZymes, (hemi)cellulases point complex nuanced degradation strategy. Conclusions Our analysis T. elucidates enzyme arsenal that fungus uses degrade lignocellulosic study provides basis future characterization potential new industrial saccharification.